FIV

Quantitative analysis of proviral and viral forms of Feline Immunodeficiency Virus (FIV) in cat and other felines (tiger, lion, leopard ...)
Detection of latent and active infections.

Reasons for testing

Symptomatic animals

 Diagnostics of FIV infections causing various clinical signs (anemia, immunosuppression, recurrent infections,...)
 Retest of an animal with a positive result with an immunologic assay
 Monitoring of blood viral load (on request, contact us)

Asymptomatic animals

 Early stage of infection (recent contact)
 Young kittens born to infected queens or with a serological positive result
 Confirmation of a positive result with an immunologic assay (speed test or in-lab assay)

Whatever the clinical status

 Confirmation of an equivocal immunologic result

Test characteristics

• quantitative RT-PCR: amplification of viral RNA and DNA
  Detection of provirus (viral genome integrated in the cat genome, necessary in the retroviral replication cycle) and virus (means viral replication)
• Limit of detection
• Quantitative analysis (on request, contact us)

Diagnostic samples

• Blood (EDTA)
• Bone marrow (EDTA)
• Blood (EDTA) and oropharyngeal cells (cytobrush or dry swab) in case of buccal disorders (stomatitis, gingivitis...)
• CSF or aqueous humor for neurological/ocular forms (uveitis) (and blood if possible)
• Tumor biopsy, organs, lymph nodes, fluids, pad biopsy… (with no formalin)

Result interpretation

• Negative result : absence of FIV or viral load lower than the limit of detection.
  This viral etiology can be excluded in symtomatic cats.
  
• Positive result : this animal is infected with FIV (the infection can possibly be latent)

Do not hesitate to contact us for sample collecting advice or help in interpreting the results.